STUDY OF BIOSORPTION USING CHLORELLA MICROALGAE FOR THE PROTECTION OF WATER RESOURCES

Main Article Content

Iroda Kayumova
Sadritdin Turabdzhanov
Davronbek Abdurakhmanov

Abstract

Under conditions of increasing anthropogenic pressure on aquatic ecosystems, particularly in industrially developed regions, the development of effective wastewater treatment methods is a key objective for water management. This paper presents the results of a study aimed at developing an environmentally safe technology for the purification of metallurgical wastewater from copper ions (Cu(II)) using the biosorption potential of unicellular green microalgae of the genus Chlorella. The tolerance and biosorption capacity of Chlorella vulgaris and Chlorella pyrenoidosa strains adapted to high copper concentrations were studied experimentally. A pilot technological scheme for the biological polishing of wastewater was developed and successfully tested under pilot-scale conditions at one of the largest industrial hubs in Central Asia – JSC "Almalyk Mining and Metallurgical Combine" (AGMK). The economic and environmental feasibility of applying Chlorella-based biosorption for reducing the technogenic load on water bodies and improving water resource quality in regions with developed metallurgical industries is demonstrated.

Article Details

Section
Geoecology
Author Biographies

Iroda Kayumova, Tashkent State Technical University

PhD student (Tashkent State Technical University, Tashkent, Uzbekistan; kayumova.iroda@inbox.ru)

Sadritdin Turabdzhanov, Tashkent State Technical University

D.t.s., Professor, Rector (Tashkent State Technical University, Tashkent, Uzbekistan; tur_sad@mail.ru)

Davronbek Abdurakhmanov, JSC "Almalyk Mining and Metallurgical Combine"

Head of the Environmental Protection Department (JSC "Almalyk Mining and Metallurgical Combine", Tashkent, Uzbekistan; d.abdurahmanov@agmk.uz)

References

Муминова Р. Н., Казимова Н. М. Проблема очистки сточных вод в Узбекистане // Молодой учёный. – 2015. – №22(102). – С. 47–48. https://moluch.ru/archive/102/23372/

Abo-Alkasem M. I., Hassan N. H., Abo Elsoud M. M. Microbial bioremediation as a tool for the removal of heavy metals // Bull Natl Res Cent. – 2023. – №47. – Р. 31. https://doi.org/10.1186/s42269-023-01006-z

Mehta S. K., Gaur J. P. Use of algae for removing heavy metal ions from wastewater: Progress and prospects // Critical Reviews in Biotechnology. – 2005. №25(3). – Р. 113–152. https://doi.org/10.1080/07388550500248571

Al-Rub, F. A. A., El-Naas, M. H., Ashour, I., Al-Marzouqi, M. Biosorption of copper on Chlorella vulgaris from single, binary and ternary metal aqueous solutions // Process Biochemistry. 2006. – №41(2). – Р. 457–464. https://doi.org/10.1016/j.procbio.2005.07.018

Aksu, Z., Dönmez, G. Binary biosorption of cadmium(II) and nickel(II) onto dried Chlorella vulgaris: Co-ion effect on mono-component isotherm parameters // Process Biochemistry. – 2006. – №41(4). – Р. 860–868. https://doi.org/10.1016/j.procbio.2005.10.025

Турабджанов С. М., Понамарева Т. В., Юсупова Д. А., Назиров З. Ш., Рахимова Л. С. Исследование сорбции ионов меди из сточных вод фосфорнокислым катионитом // Химическая безопасность. – 2018. – №2(2). – С. 173–182. https://doi.org/10.25514/CHS.2018.2.14115

Jiang, Q., Chen, H., Fu, Z., Fu, X., Wang, J., Liang, Y., Yin, H., Yang, J., Jiang, J., Yang, X., Wang, H., Liu, Z., Su, R. Current Progress, Challenges and Perspectives in the Microalgal-Bacterial Aerobic Granular Sludge Process: A Review // Int J Environ Res Public Health. – 2022. – №19(21). – Р. 13950. https://doi.org/10.3390/ijerph192113950

Oruganti, R. K., Katam, K., Show, P. L., Gadhamshetty, V., Upadhyayula, V. K. K., Bhattacharyya, D. A comprehensive review on the use of algal-bacterial systems for wastewater treatment with emphasis on nutrient and micropollutant removal // Bioengineered. – 2022. – №13(4). – Р. 10412-10453. https://doi.org/10.1080/21655979.2022.2056823

Thiruchchelvam, T., Sathasivan, A. Can microalgae-bacteria system started with Chlorella vulgaris treat municipal wastewater without mechanical aeration? // Case Studies in Chemical and Environmental Engineering. – 2025. – №11. – Р. 101236. https://doi.org/10.1016/j.cscee.2025.101236

Chojnacka, K. Using biosorption to enrich the biomass of Chlorella vulgaris with microelements to be used as mineral feed supplement // World Journal of Microbiology and Biotechnology. – 2007. – №23(7). – Р. 1139–1147. https://doi.org/10.1007/s11274-006-9344-9

Al-Qunaibit, M. H. Divalent Cu, Cd, and Pb biosorption in mixed solvents // Bioinorganic Chemistry and Applications. – 2009. – Р. 561091. https://doi.org/10.1155/2009/561091

Ahmad, A., Bhat, A. H., Buang, A. Biosorption of transition metals by freely suspended and Ca-alginate immobilised with Chlorella vulgaris: Kinetic and equilibrium modeling // Journal of Cleaner Production. – 2018. – №171. – Р. 1361–1375. https://doi.org/10.1016/j.jclepro.2017.10.069

Cheng, J., Yin, W., Chang, Z., Lundholm, N., Jiang, Z. Biosorption capacity and kinetics of cadmium(II) on live and dead Chlorella vulgaris // Journal of Applied Phycology. – 2017. – №29(1). – Р. 211–221. https://doi.org/10.1007/s10811-016-0916-2

Wanta, K. C., Miryanti, A., Kristijarti, A. P. Biosorption of Cu(II) ions using living microalgae Chlorella sp.: Effects of microalgae concentration, salinity, and light color // International Journal of Technology. – 2023. – №14(1). – Р. 291–319. https://doi.org/10.14716/ijtech.v14i1.4995

Edris, G., Alhamed, Y., Alzahrani, A. Biosorption of cadmium and lead from aqueous solutions by Chlorella vulgaris biomass: Equilibrium and kinetic study // Arabian Journal for Science and Engineering. – 2014. – №39(1). – Р. 87–93. https://doi.org/10.1007/s13369-013-0820-x

Moreira, V. R., Lebron, Y. A. R., Freire, S. J., Santos, L. V. S., Palladino, F., Jacob, R. S. Biosorption of copper ions from aqueous solution using Chlorella pyrenoidosa: Optimization, equilibrium and kinetics studies // Microchemical Journal. – 2019. – №145. – Р. 119–129. https://doi.org/10.1016/j.microc.2018.10.027

Mahlangu, D., Mphahlele, K., De Paola, F., Mthombeni, N. H. Microalgae-Mediated Biosorption for Effective Heavy Metals Removal from Wastewater: A Review // Water. – 2024. – №16(5). – Р. 718. https://doi.org/10.3390/w16050718

Almomani, F., Bhosale, R. R. Bio-sorption of toxic metals from industrial wastewater by algae strains Spirulina platensis and Chlorella vulgaris: Application of isotherm, kinetic models and process optimization // Science of The Total Environment. – 2021. – №755(2). – Р.142654. https://doi.org/10.1016/j.scitotenv.2020.142654

Kayumova, I. K., Sidicov, S., Egamberdiev, E. A., Turabjanov, S. M. Biosorption of Cu(II) ions from metal plant wastewater using Chlorella // Multidisciplinary Journal of Science and Technology. – 2025. – №5(6). – Р. 2398–2402.

СанПиН РУз №0211-06. Гигиенические критерии и контроль качества воды централизованных систем хозяйственно-питьевого водоснабжения населения Узбекистана. – Ташкент, 2006.

Andersen, R. A. (ed.). Algal Culturing Techniques. – Burlington: Elsevier Academic Press, 2005.

UTEX Culture Collection of Algae. Bristol Medium. https://utex.org/products/bristol-medium (дата обращения: 10.03.2025).

Johnson, H. L., Stauber, J. L., Adams, M., Jolley, D. F. Copper and zinc tolerance of two tropical microalgae after copper acclimation // Environ Toxicol. – 2007. – №22(3). – Р. 234–244.

Mechanism of Biological Transport and Transformation of Copper, Cadmium, and Zinc in Water by Chlorella // Water. – 2024. – №16(13). – Р. 1906. https://www.mdpi.com/2073-4441/16/13/1906

Aksu, Z. Absorption and Adsorption of Heavy Metals by Microalgae // Handbook of Microalgal Culture: Applied Phycology and Biotechnology. – Chichester: Wiley-Blackwell, 2013. – Р. 602–622. https://doi.org/10.1002/9781118567166.ch32

Volesky, B. Biosorption of Heavy Metals. – Boca Raton: CRC Press, 1990.

Wilde, E. W., Benemann, J. R. Biosorption of Heavy Metals by Microalgae in Batch and Continuous Systems // Wastewater Treatment with Algae. – Berlin; Heidelberg: Springer, 1998. – Р. 37–53. https://link.springer.com/chapter/10.1007/978-3-662-10863-5_3

Zeraatkar, A. K., Ahmadzadeh, H., Talebi, A. F., Moheimani, N. R., McHenry, M. P. Biosorption: An Interplay between Marine Algae and Potentially Toxic Elements—A Review // Marine Drugs. – 2016. – V. 14, № 4. – Art. 65. https://doi.org/10.3390/md14040065

ГОСТ 31870-2012. Вода питьевая. Определение содержания элементов методами атомной спектрометрии. – Введ. 2013-07-01. – М.: Стандартинформ, 2013. https://docs.cntd.ru/document/1200097409

ГОСТ Р 54276-2010. Вода. Методы определения меди. – Введ. 2012-07-01. – М.: Стандартинформ, 2011. https://allgosts.ru/13/060/gost_r_54276-2010

Standard Operating Procedures for Analytical Methods and Data Collection in Support of Pilot-Scale Cultivation of Microalgae / EnAlgae Consortium. – Swansea: EnAlgae Project, 2015. https://repository.oceanbestpractices.org/bitstream/handle/11329/1283/WP1A3.01%20EnAlgae%20SOP%20report-v10%20FINAL.pdf